From the other replies in the thread, it seems like there is a contingent of people who believe that if someone doesn't take the first "no" as a final "no" then they are no-true-asker, and instead is just an asshole.
In my life I've never met an asker who took no as final. Maybe they said they respected it, but at least they wanted to understand why not and what could they do to help me make it a yes. At that point you're not just asking, you're wasting my time.